Journal: Cell Reports Medicine

Article Title: Systemic and mucosal immune signatures of protection against SARS-CoV-2 transmission in humans

doi: 10.1016/j.xcrm.2025.102505

Figure Lengend Snippet: Longitudinal antibody response to SARS-CoV-2, HCoV-OC43, and HCoV-229E proteins in the systemic compartment Serum from participants was analyzed at baseline and at days 3, 7, and 28 post-enrollment. (A) IgG, IgM, and IgA response for SARS-CoV-2 full-length protein and S1, S2, and RBD domains according to groups (index, positive cases, negative cases). Dots represent the GMT, and bars indicate 95% CI. (B) IgG, IgM, and IgA response for HCoV-OC43 full-length protein and S1 and S2 domains according to groups (index, positive cases, negative cases). Dots represent the GMT, and bars indicate 95% CI. (C) IgG, IgM, and IgA response for HCoV-229E full-length protein according to groups (index, positive cases, negative cases). Dots represent the GMT, and bars indicate 95% CI. ∗ p ≤ 0.05. Data on antibody were compared by multiple comparison analysis (Kruskal-Wallis test); if multiple comparison was statistically significant (∗ p ≤ 0.05), a pairwise comparison was performed and the Bonferroni correction for multiple tests was applied. Data on two-by-two comparison are shown in .

Article Snippet: 229e full-length spike protein , Sino Biological , Cat#40605-V08B.

Techniques: Comparison

Journal: Cell Reports Medicine

Article Title: Systemic and mucosal immune signatures of protection against SARS-CoV-2 transmission in humans

doi: 10.1016/j.xcrm.2025.102505

Figure Lengend Snippet: Binomial logistic regression of preexisting SARS-CoV-2, HCoV-OC43, and HCoV-229E antibody levels and the probability of infection upon SARS-CoV-2 exposure in the CIDS cohort (A) Violin plot of IgG, IgM, and IgA baseline antibody titers (log10 AUC) for SARS-CoV-2-S, HCoV-OC43-S, and HCoV-229e-S full-length proteins in index, positive cases, and negative cases groups. Median (continuous black line), and IQR (Q1–Q3) values (discontinued lines) are represented. Pairwise comparisons adjusted by the Bonferroni correction for multiple test has been performed, and significance of ∗ p < 0.05 is shown. (B–D) Scatterplot diagrams showing the inverse relationship between the predicted probability of infection (PP infection, %) and immunoglobulin levels for the indicated SARS-CoV-2 antigens: S (full-length), S1, RBD, and S2; (C) HCoV-OC43: S (full-length), S1, and S2; and (D) HCoV-22E S (full-length). Logistic regression analysis has been performed for each variable individually, and significance of ∗ p < 0.05 is shown.

Article Snippet: 229e full-length spike protein , Sino Biological , Cat#40605-V08B.

Techniques: Infection

Journal: Cell Reports Medicine

Article Title: Systemic and mucosal immune signatures of protection against SARS-CoV-2 transmission in humans

doi: 10.1016/j.xcrm.2025.102505

Figure Lengend Snippet: Multi-assay modeling of mucosal signatures of protection against SARS-CoV-2 infection Nasopharyngeal swab samples from participants were analyzed at baseline and at days 3, 7, and 28 post-enrollment. (A–H) IgG, IgM, IgA, and secretory IgA (sIgA) response for (A) SARS-CoV-2 S and (B) HCoV-OC43 S proteins according to groups (index, positive cases, and negative cases) on the upper respiratory tract. Dots represent the GMT, and bars indicate 95% CI. Fold change antibody titers against (C) SARS-CoV-2 and (D) HCoV-OC43 S protein represented as boxplot. Box indicates IQR (Q1–Q3), with horizontal line at median and whiskers representing minimum and maximum. Binomial logistic regression of pre-existing (E) SARS-CoV-2 and (F) HCoV-OC43 antibody levels and the probability of SARS-CoV-2 infection. Scatterplot diagrams show the inverse relationship between the PP infection % and anti-SARS-CoV-2 S IgG, IgM, IgA, and sIgA mucosal levels. Multivariate logistic regression model analyzing the potential protection of (G) anti-SARS-CoV-2 S and (H) anti-HCoV-OC43 S IgG, IgM, IgA, and sIgA mucosal antibody levels at enrollment. (I) Multivariate logistic regression model analyzing the potential protection of mucosal anti-S IgG SARS-CoV-2 and anti-S IgA HCoV-OC43 antibody levels at enrollment from SARS-CoV-2 infection. (J) Mixed multivariate logistic regression analysis of the potential protection of systemic (SARS-CoV-2 S1 IgG and HCoV-OC43 S2 IgM) and mucosal (SARS-CoV-2 S IgG and HCoV-OC43 S IgA) antibody levels at enrollment for SARS-CoV-2 infection. Data on antibody were compared by multiple comparison analysis (Kruskal-Wallis test); if multiple comparison was statistically significant (∗ p ≤ 0.05), a pairwise comparison was performed and the Bonferroni correction for multiple tests was applied. Data on two-by-two comparison are shown in and .

Article Snippet: 229e full-length spike protein , Sino Biological , Cat#40605-V08B.

Techniques: Infection, Comparison